γ -Glutamyltransferase (GGT) Assay Kit/Test Kit/Diagnostic Reagents

The γ-Glutamyltransferase (GGT) assay kit is intended for the accurate and direct quantitative measurement of GGT activity in human plasma. GGT measurement is useful in the diagnosis of hepatobiliary disease.

Test principle

In this assay kit, soluble L-γ-glutamyl-3-carboxyl-p-nitroaniline (Glupac) serves as a substrate, and glycyglycine is designed to be a receptor of γ-glutamyl. γ-glutamyl can be transferred to glycyglycine molecules when catalyzed by GGT, producing yellow-colored 3-hydroxyl-p-nitroaniline. The reaction can induce an increase of absorbance at 405 nm, and the increasing rate is proportional to the activity of GGT.
 
Product Features
  Linear correlation coefficient ∣r∣≥ 0.990
Linear deviation ≤ ± 10.00% Changing rate of absorbance of blank ΔmA/min ≥ 0.05 while testing the specific GGT sample of activity of 100 U/L. Relative deviation is within ±10%. Intra CV% ≤ 6.0%; inter CV% ≤8.0%.     

Technical Parameters
  Reference range of GGT activity: 5 - 50 U/L.

Packing Specifications

R1: 2×60 ml, R2: 2×15 mL
R1: 4×60 ml, R2: 4×15 mL
R1: 1×40 ml, R2: 1×10 mL (Sample)

Storage and stability
Unopened: Store assay kits at 2 - 8ºC protected from light. Stable to expiration date printed on individual vials (for 15 months).
Opened: Store assay kits at 2 - 8ºC protected from light. Stable for 1 month if kept capped from original container and free from contamination.
Do not freeze the assay kit.
  The γ-Glutamyltransferase (GGT) assay kit is intended for the accurate and direct quantitative measurement of GGT activity in human plasma. GGT measurement is useful in the diagnosis of hepatobiliary disease.

Test principle

In this assay kit, soluble L-γ-glutamyl-3-carboxyl-p-nitroaniline (Glupac) serves as a substrate, and glycyglycine is designed to be a receptor of γ-glutamyl. γ-glutamyl can be transferred to glycyglycine molecules when catalyzed by GGT, producing yellow-colored 3-hydroxyl-p-nitroaniline. The reaction can induce an increase of absorbance at 405 nm, and the increasing rate is proportional to the activity of GGT.
 
Product Features
  Linear correlation coefficient ∣r∣≥ 0.990
Linear deviation ≤ ± 10.00% Changing rate of absorbance of blank ΔmA/min ≥ 0.05 while testing the specific GGT sample of activity of 100 U/L. Relative deviation is within ±10%. Intra CV% ≤ 6.0%; inter CV% ≤8.0%.     

Technical Parameters
  Reference range of GGT activity: 5 - 50 U/L.

Packing Specifications

R1: 2×60 ml, R2: 2×15 mL
R1: 4×60 ml, R2: 4×15 mL
R1: 1×40 ml, R2: 1×10 mL (Sample)

Storage and stability
Unopened: Store assay kits at 2 - 8ºC protected from light. Stable to expiration date printed on individual vials (for 15 months).
Opened: Store assay kits at 2 - 8ºC protected from light. Stable for 1 month if kept capped from original container and free from contamination.
Do not freeze the assay kit.
 

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