Introduction to the use of Vector Immunohistochemical Pen (H-4000)

Vector immunohistochemical pen, ImmEdge Hydrophobic Barrier Pen, also known as ImmEdge Pen, super immunohistochemical pen, suitable for various immunohistochemical staining experiments of glass sections, such as PAP method, ABC method, immunofluorescence, frozen section and In situ hybridization technology can reduce the amount of antibodies and reagents, avoid liquid flow and diffusion during dyeing, and increase the speed of operation. Currently used in hospitals, cancer treatment research centers, animal and plant quarantine centers, colleges and universities, pathology laboratories, cell laboratory, chemical laboratory, immunohistochemical staining laboratory and other research laboratories Check the specimen.

Vector H-4000 immunohistochemical pen overcomes the common ills of common self-made immunohistochemical pens, immunohistochemical pen substitutes, and common immunohistochemical pens, such as difficult to write, unfavorable oil or excessive oil, polluted glass Sheet, poor water barrier, less loops, etc. Since the successful development, it has won unanimous praise from the majority of users and is exported to countries all over the world. Both US Invitrogen and Millipore recommend the use of Vector's immunohistochemical pen.

Invitrogen recommends "Invitrogen can only advise that you dispose of the material in compliance with all applicable local, ... If your protocol requires the use of a PAP pen, we recommend the ImmEdge Hydrophobic Barrier Pen(Cat. # H-4000) from Vector Labs. ...
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First dewax the tissue section, then wipe the liquid on the edge of the tissue on the slide with tissue paper, then use the immunohistochemical pen to draw a circle around the specimen on the slide or draw a line on the edge of the tissue, dry 10-15 seconds, then slide the slide in PBS.