How to choose the right high sensitivity RFP (DsRed) tag antibody?

Red fluorescent protein (RFP) is a protein that is isolated from the Pacific Ocean and an anemone (Discosomasp) that emits red fluorescence under ultraviolet light. High fluorescence conversion efficiency in cells. It is a naturally occurring fluorescent protein with the longest excitation wavelength and emission wavelength. The protein encoded by RFP gene is composed of 225 amino acids with a relative molecular mass (Mr) of 25.9 ku. RFP is widely used in animals, plants and yeast. Such as the expression of genes in eukaryotic cells, the application of their genes in prokaryotic cells has also received increasing attention.

How to select the appropriate RFP (DsRed) tag antibody?

When we choose RFP (DsRed) antibodies, we mainly need according to our own needs, especially for experimental applications. In addition, the more types of applications after an antibody is detected, the greater the choice in use; while the monoclonal antibodies from mouse sources generally have better specificity, stability, and potency than polyclonal antibodies. In addition, the titer of the antibody is also an important aspect to examine the cost performance of the antibody, which is equivalent to the dilution ratio in practical use. A high titer of 100 μl of RFP antibody (if the dilution ratio of WB is 1:5000, the final solution is equivalent to 500 ml), 1 ml of RFP antibody with lower potency (if the dilution ratio of WB test is 1:200) The final use solution is equivalent to 200ml).

Product name and description Item number product manual
Anti-RFP Tag Mouse Monoclonal Antibody (9D1) A02120 Details

Why recommend the Abbkine brand of red fluorescent protein RFP (DsRed) tag antibody (clone 9D1 )?

At present, the RFP-labeled antibodies available on the market are domestically packaged or originated. The domestically-packaged RFP-labeled antibodies have insufficient quality stability, and after being packaged, their titers are also lost. Some RFP-labeled antibodies originating in domestic laboratories have better detection activity and are difficult to control in batch stability, while only a small number of native antibodies in China are also polyclonal antibodies. As for the imported brands that supply RFP-labeled antibodies, there are not many imported brands. Only Abcam, Sigma and other brands can provide RFP-labeled antibodies, but they are far less expensive than Abbkine products.

"RFP (DsRed) tag antibody comprehensive comparison"

Brand Item number App types Price / specification
Abbkine A02120 WB: (1:5000) 605/50ul 1800/200ul 6020/1ml
Abcam Ab65856 WB: (1:1000)

IF : (self-exploration)

4340/100ul

Therefore, we recommend Abbkine's Anti-RFP Tag Mouse Monoclonal Antibody (9D1). Like Abcam, Abbkine's RFP antibody immunogen is a recombinant RFP full-length protein. After continuous screening and optimization, the best cells are finally obtained. Strain (9D1). As a production brand in the United States, Abbkine provides OEM services to a number of internationally renowned brands, so its price is low and its quality has undergone market testing. This antibody can be used for WB.

Antibody validation results (A, 1:2000 dilution; B, 1:3000 dilution; C, 1:5000 dilution)

Product name and description Item number product manual
Anti-RFP Tag Mouse Monoclonal Antibody (9D1) A02120 Details

Abbkine also offers 1ml large packaging and larger custom-made RFP antibodies, making it ideal for routine RFP immunoassays. For the convenience of customers, Abbkine is available in stock in China. Abbkine provides global product quality tracking and after-sales service and protection for the Chinese market. If you have any problems during use, please contact Abbkine local agent or Aimei Technology Co., Ltd. directly.

The American Abbkine brand is a brand that provides cost-effective immunology and cytology research products and solutions. Amy Jie introduced it to China as its regional agent in China, and will provide customers with the most cost-effective routine immunological reagents, such as A tag antibody, an internal reference antibody, a conventional primary antibody, and a full range of secondary antibodies, antibody detection substrates, and related kits. At the same time, as a Chinese agent or regional agent of many international famous brands, Aimei can provide you with a series of laboratory solutions. If you are interested in the above products, please call the free customer line 400-6800-868 to contact Aimei Technology Co., Ltd. for product information and complete experimental solutions.

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