Product Name: GelRedTM Nucleic Acid Gel Dye, 10,000X in DMSO
Product number: 41002
Packing specification: 0.5 mL / 0.05 mL
Storage and disposal methods: GelRed 10,000X in DMSO can be stored at room temperature for more than one year. Although not required, GelRed can still be stored for a long time in low temperature and dark environments. However, under normal indoor lighting conditions, the stain can be safely handled.
Application: GelRed® is a red fluorescent nucleic acid stain with gel staining properties and designed to replace the highly toxic dye ethidium bromide (EB). Because GelRed has the same spectral characteristics as EB (Figure 1), you can replace EB with GelRed without changing any imaging system.
If you are currently using SYBR (such as SYBR Green 1/SYBR Gold) stains and using a UV transilluminator to observe the gel, you can replace the SYBR stain with GelRed without replacing the existing ones. SYBR light film.
However, GelRed® cannot be fully excited at 488 nm or similar visible light. If required, we recommend that you use our GelGreen(Cat# 41004) stain with the same sensitivity as SYBR Green 1, but its stability and Reliability is far better than the latter.
GelRed can be used for both precast gel staining and post gel staining. Generally, post-staining can obtain more sensitive characteristics than pre-staining, and can eliminate the possibility that the dye will have any influence on the separation of nucleic acid bands during electrophoresis. However, pre-dyeing is easier and more economical than post-dyeing because the pre-dyeing does not require an additional coloring process and the dye usage is less. Therefore, if sensitivity and strip definition are not a problem, pre-dyeing is preferred. We strongly recommend that you try two staining methods to choose the best staining method for your needs.
In summary, GelRed exceeds SYBR or EB in terms of performance and operability. In general, the most striking feature of GelRed is its high sensitivity and stability under a variety of conditions. In addition, like GelGreen(R) and EvaGreen(R), GelRed(R) has a very low ability to induce mutations relative to EB or SYBR. Toxicity testing reports from authoritative laboratories can be downloaded from the Biotium website ().
Cat#41000 (GelRed® Nucleic Acid Gel Stain, 10,000X in DMSO) is a concentrated GelRed® solution. For pre-dyeing, it can be diluted 10,000 times and used. For post-dyeing, it is recommended to dilute 3,300 times and use it. See the specific steps.
Other types of GelRed are: GelRed 10,000X in H2O, 0.5mL (cat# 41003); GelRed 10,000X in DMF, 0.5mL (cat# 41000); and GelRed 3X in H2O, 4L (#cat 41001) .
Steps:
1) Pre-staining method for DNA staining (precast gel staining)
1.1. Prepare the agarose gel solution using standard methods
1.2. Dissolve the GelRedTM 10,000X in DMSO (41000) reagent in 1:30 in agarose gel solution, and mix the stain with the gel solution by vortexing, stirring or shaking. (Example: Add 5uL of GelRedTM Reagent to a 50mL gel solution).
Due to its excellent thermal stability, GelRedTM can be added directly to a high temperature gel solution without waiting for the gel solution to cool before adding. It is also possible to mix the GelRedTM reagent with the gel powder beforehand, then add the buffer solution you are using, and use a conventional method for preparing agarose gel by microwave or other heating method.
Note: GelRedTM is suitable for all common electrophoresis buffer solutions.
1.3. The gel solution is poured and allowed to solidify. The remaining gel solution can be stored and reheated to cast another gel. Thanks to the good hydrolytic stability of GelRedTM (see Figure 2), you can prepare a large number of GelRedTM gels for storage. To avoid the formation of agglomerates, it is recommended to pre-form the gel 4 ï‚° C for refrigeration.
1.4. Standard method Loading sample running glue Note: Using this agarose colloid with pre-doped dye, it is not easy to add too many DNA samples at a time, otherwise it is easy to cause saturation, you can do multiple DNA markers of different concentrations. (marker) to determine the optimal amount of DNA loading.
1.5. Nucleic acid color development was performed using a standard fluoroscope (302 nm) and photographed with Polaroid 667 film and EB filters. Since the fluorescence is in the red light region, the SYBR or GelStar filter can also be used for photographing, giving the same good results. (See Figure 1 for GelRedTM emission and excitation spectra)
Note: If there is always a tailing or the strip cannot be separated, you can use the post-staining method to stain the DNA to see if the problem is related to the stain. If the problem of post-dyeing still exists, the problem is not related to the stain. Try: Reduce the agarose content; Reduce the loading of nucleic acids; Improve the test methods and techniques.
In general, GelRed or GelGreen have a lower impact on DNA movement than SYBR Green I. As shown in Figure 3.
2) Post-staining method for DNA staining (Post Gel Staining)
2.1. Run the glue using standard methods
2.2. The GelRedTM 10,000X in DMSO (41000) reagent was diluted approximately 3,300 times with H2O containing 0.1 M NaCl to make a 3X staining solution. (Example: 15 uL of GelRedTM 10,000X reagent and 5 mL of NaCl were added to 45 mL of H2O).
Note: GelRedTM 1X staining solution can also be used for post-staining, but its sensitivity is lower than 3X staining solution.
2.3. Carefully place the gel in a suitable container, such as a polypropylene container. Slowly add enough 3X staining solution to immerse the gel.
2.4. Gently shake the gel plate at room temperature for a minimum of 30 minutes depending on the thickness of the gel plate, the concentration of agarose or polyacrylamide, and the length of the DNA. The thicker the gel or the higher the concentration of agarose, the longer it takes to stain.
Note: The staining solution can be reused for at least 2-3 times. If it is not used immediately, it is recommended to refrigerate the used staining solution.
2.5. The stained gel was observed through a standard fluoroscope (302 nm) and photographed with a Polaroid 667 and EB filter. Similarly, SYBR® or GelStar® filters can also be used for photographing, giving equally good results.
Figure 1. Excitation spectra (left) and emission spectra (right) of GelRedTM binding to dsDNA in TBE buffer.
Figure 2. Comparison of GelRedTM and SYBR Gold stability. GelRed and SYBR Gold 1X TBE gel staining solutions have normal absorbance at 500 nm and 488 nm for long periods of time at room temperature. GelRedTM and SYBR Gold initial absorbance values ​​were 0.029 and 0.051, respectively.
Figure 3. Diagram of the distance traveled by dsDNA and its fragment size. GelRed post-staining, GelRed, GelGreen, SYBRGreen I were used for pre-staining.
*GelRedTM products and their use are protected by US and international patents
** SYBR is a registered trademark of Molecular Probes, Inc., and GelStar is a registered trademark of FMC. Toxicity: Mutation tests were initially performed on GelRedTM using a commercial mutagenicity test, GelRedTM in the absence or presence of a rat liver extract. The frameshift indicator strain TA98 showed very weak mutagenicity. Further safety tests have shown that GelRedTM also exhibits very safe experimental results. Since it is recommended for chemicals, especially for nucleic acid-bound chemicals, care should be taken during operation.
Toxicity testing reports from authoritative laboratories can be downloaded from the Biotium website ().
Treatment: Add ~100mL of bleach (house bleach) to each gallon (~4L) of discarded dye solution, fully react for more than 8 hours and then pour into the sewer. For the pre-made glue treatment, it can be fully dried and then treated as normal waste.
First aid method: This stain is potentially harmful. Avoid long periods of time or repeated exposure to light. Avoid contact with eyes, skin or clothing and wash thoroughly after handling. Rinse the infected area with plenty of water for 15 minutes immediately after eye or skin contact, and seek medical attention immediately. In case of aspiration or swallowing, immediately move to fresh air and seek medical attention immediately.
Product number: 41002
Packing specification: 0.5 mL / 0.05 mL
Storage and disposal methods: GelRed 10,000X in DMSO can be stored at room temperature for more than one year. Although not required, GelRed can still be stored for a long time in low temperature and dark environments. However, under normal indoor lighting conditions, the stain can be safely handled.
Application: GelRed® is a red fluorescent nucleic acid stain with gel staining properties and designed to replace the highly toxic dye ethidium bromide (EB). Because GelRed has the same spectral characteristics as EB (Figure 1), you can replace EB with GelRed without changing any imaging system.
If you are currently using SYBR (such as SYBR Green 1/SYBR Gold) stains and using a UV transilluminator to observe the gel, you can replace the SYBR stain with GelRed without replacing the existing ones. SYBR light film.
However, GelRed® cannot be fully excited at 488 nm or similar visible light. If required, we recommend that you use our GelGreen(Cat# 41004) stain with the same sensitivity as SYBR Green 1, but its stability and Reliability is far better than the latter.
GelRed can be used for both precast gel staining and post gel staining. Generally, post-staining can obtain more sensitive characteristics than pre-staining, and can eliminate the possibility that the dye will have any influence on the separation of nucleic acid bands during electrophoresis. However, pre-dyeing is easier and more economical than post-dyeing because the pre-dyeing does not require an additional coloring process and the dye usage is less. Therefore, if sensitivity and strip definition are not a problem, pre-dyeing is preferred. We strongly recommend that you try two staining methods to choose the best staining method for your needs.
In summary, GelRed exceeds SYBR or EB in terms of performance and operability. In general, the most striking feature of GelRed is its high sensitivity and stability under a variety of conditions. In addition, like GelGreen(R) and EvaGreen(R), GelRed(R) has a very low ability to induce mutations relative to EB or SYBR. Toxicity testing reports from authoritative laboratories can be downloaded from the Biotium website ().
Cat#41000 (GelRed® Nucleic Acid Gel Stain, 10,000X in DMSO) is a concentrated GelRed® solution. For pre-dyeing, it can be diluted 10,000 times and used. For post-dyeing, it is recommended to dilute 3,300 times and use it. See the specific steps.
Other types of GelRed are: GelRed 10,000X in H2O, 0.5mL (cat# 41003); GelRed 10,000X in DMF, 0.5mL (cat# 41000); and GelRed 3X in H2O, 4L (#cat 41001) .
Steps:
1) Pre-staining method for DNA staining (precast gel staining)
1.1. Prepare the agarose gel solution using standard methods
1.2. Dissolve the GelRedTM 10,000X in DMSO (41000) reagent in 1:30 in agarose gel solution, and mix the stain with the gel solution by vortexing, stirring or shaking. (Example: Add 5uL of GelRedTM Reagent to a 50mL gel solution).
Due to its excellent thermal stability, GelRedTM can be added directly to a high temperature gel solution without waiting for the gel solution to cool before adding. It is also possible to mix the GelRedTM reagent with the gel powder beforehand, then add the buffer solution you are using, and use a conventional method for preparing agarose gel by microwave or other heating method.
Note: GelRedTM is suitable for all common electrophoresis buffer solutions.
1.3. The gel solution is poured and allowed to solidify. The remaining gel solution can be stored and reheated to cast another gel. Thanks to the good hydrolytic stability of GelRedTM (see Figure 2), you can prepare a large number of GelRedTM gels for storage. To avoid the formation of agglomerates, it is recommended to pre-form the gel 4 ï‚° C for refrigeration.
1.4. Standard method Loading sample running glue Note: Using this agarose colloid with pre-doped dye, it is not easy to add too many DNA samples at a time, otherwise it is easy to cause saturation, you can do multiple DNA markers of different concentrations. (marker) to determine the optimal amount of DNA loading.
1.5. Nucleic acid color development was performed using a standard fluoroscope (302 nm) and photographed with Polaroid 667 film and EB filters. Since the fluorescence is in the red light region, the SYBR or GelStar filter can also be used for photographing, giving the same good results. (See Figure 1 for GelRedTM emission and excitation spectra)
Note: If there is always a tailing or the strip cannot be separated, you can use the post-staining method to stain the DNA to see if the problem is related to the stain. If the problem of post-dyeing still exists, the problem is not related to the stain. Try: Reduce the agarose content; Reduce the loading of nucleic acids; Improve the test methods and techniques.
In general, GelRed or GelGreen have a lower impact on DNA movement than SYBR Green I. As shown in Figure 3.
2) Post-staining method for DNA staining (Post Gel Staining)
2.1. Run the glue using standard methods
2.2. The GelRedTM 10,000X in DMSO (41000) reagent was diluted approximately 3,300 times with H2O containing 0.1 M NaCl to make a 3X staining solution. (Example: 15 uL of GelRedTM 10,000X reagent and 5 mL of NaCl were added to 45 mL of H2O).
Note: GelRedTM 1X staining solution can also be used for post-staining, but its sensitivity is lower than 3X staining solution.
2.3. Carefully place the gel in a suitable container, such as a polypropylene container. Slowly add enough 3X staining solution to immerse the gel.
2.4. Gently shake the gel plate at room temperature for a minimum of 30 minutes depending on the thickness of the gel plate, the concentration of agarose or polyacrylamide, and the length of the DNA. The thicker the gel or the higher the concentration of agarose, the longer it takes to stain.
Note: The staining solution can be reused for at least 2-3 times. If it is not used immediately, it is recommended to refrigerate the used staining solution.
2.5. The stained gel was observed through a standard fluoroscope (302 nm) and photographed with a Polaroid 667 and EB filter. Similarly, SYBR® or GelStar® filters can also be used for photographing, giving equally good results.
Figure 1. Excitation spectra (left) and emission spectra (right) of GelRedTM binding to dsDNA in TBE buffer.
Figure 2. Comparison of GelRedTM and SYBR Gold stability. GelRed and SYBR Gold 1X TBE gel staining solutions have normal absorbance at 500 nm and 488 nm for long periods of time at room temperature. GelRedTM and SYBR Gold initial absorbance values ​​were 0.029 and 0.051, respectively.
Figure 3. Diagram of the distance traveled by dsDNA and its fragment size. GelRed post-staining, GelRed, GelGreen, SYBRGreen I were used for pre-staining.
*GelRedTM products and their use are protected by US and international patents
** SYBR is a registered trademark of Molecular Probes, Inc., and GelStar is a registered trademark of FMC. Toxicity: Mutation tests were initially performed on GelRedTM using a commercial mutagenicity test, GelRedTM in the absence or presence of a rat liver extract. The frameshift indicator strain TA98 showed very weak mutagenicity. Further safety tests have shown that GelRedTM also exhibits very safe experimental results. Since it is recommended for chemicals, especially for nucleic acid-bound chemicals, care should be taken during operation.
Toxicity testing reports from authoritative laboratories can be downloaded from the Biotium website ().
Treatment: Add ~100mL of bleach (house bleach) to each gallon (~4L) of discarded dye solution, fully react for more than 8 hours and then pour into the sewer. For the pre-made glue treatment, it can be fully dried and then treated as normal waste.
First aid method: This stain is potentially harmful. Avoid long periods of time or repeated exposure to light. Avoid contact with eyes, skin or clothing and wash thoroughly after handling. Rinse the infected area with plenty of water for 15 minutes immediately after eye or skin contact, and seek medical attention immediately. In case of aspiration or swallowing, immediately move to fresh air and seek medical attention immediately.
The Nursing Bed is a Hospital Bed specially designed for patients with reduced mobility during Home Care. The main purpose of the Elderly Home Care Bed is to facilitate nursing staff and facilitate patient recovery.
Home nursing beds can be divided into two categories: manual nursing beds and electric nursing beds according to the operation mode.
Home manual nursing beds can be clearly divided into manual two-function nursing beds, manual three-function nursing beds, manual five-function nursing beds, and manual multi-function nursing beds according to the number of shaking hands.
Nursing Bed,Nursing Elderly Bed Care,Elderly Home Care Bed,Metal Home Care Bed
Haloxylon Ammodendron Medical Equipment Co., Ltd. , https://www.haloxylonmedical.com