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The High Pure FFPET RNA Isolation Kit is based on the extraction principle of the spin column method and performs high-efficiency DNase digestion directly on the column to easily remove residual DNA in an integrated experimental procedure. |
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| ![]() | Figure 1: Extraction to obtain a broad range of RNA fragments, FFPET RNA extract fragments range in length from 100-4000 bp
Total RNA was extracted from 5um FFPET samples of breast cancer tissue using different methods in 3. The green line shows that there are very few long RNA fragments obtained by this method, mainly RNA-degraded fragments. The gray lines show that the RNA extract covers a wide range of fragments, but there is a large bias in the distribution of short-fragment RNA. RESULTS: The extraction product (blue line) of the Roche High Pure FFPET RNA Isolation Kit covered the entire fragment distribution more evenly. | ![]()
Figure 2: High RNA yields can be extracted from both recent and long-term FFPET samples |
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A) The bar graph shows the average yield of RNA extracted from colon cancer tissue and normal connective tissue 10um FFPET sections (n=24). The sample retention time is <6 months and >5 years, respectively.
RESULTS: The Roche High Pure FFPET RNA Isolation Kit achieved higher yields of RNA extraction products.
B) shows the leftmost two columns of the histogram of Figure A, the RNA yield of each of the 24 replicate samples. Twenty-four replicates were taken from serial sections of the tissue to avoid differences in nucleic acid content from different tissue regions.
RESULTS: In routine studies, there were differences in nucleic acid yields for different FFPET sections of the same tissue sample. But for the same sample, the Roche High Pure FFPET RNA Isolation Kit always yields higher yields of RNA extraction products. |
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| Figure 3: Extraction of high quality RNA from samples to improve RT-aPCR detection sensitivity. As a nucleic acid template in a qPCR experiment, high quality RNA, that is, good nucleic acid yield, purity, and fragment size distribution, is required, and the expression of the template gene can be detected with high sensitivity and unbiasedness. RNA was extracted from different xenograft sections of 3, 150 ng of total RNA was taken, and Transcriptor Universal cDNA Master was used for reverse transcription reaction. 10 ng of cDNA product was amplified by LightCycler 480 Real-Time PCR Instrument to detect ALAST gene expression. RESULTS: The RNA template (n=8) obtained by High Pure FFPET RNA Isolation Kit was able to obtain higher detection sensitivity in RT-qPCR experiments. |
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product information |
product | Catalog number | Packing specification | High Pure FFPET RNA Isolation Kit | 06 650 775 001 | 50 extraction reactions |
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