Exosomal microbubbles serve as important mediators of intercellular signaling, and more and more studies have revealed their clinical value as biomarkers of major diseases. At present, the mainstream analysis method of exosome microbubbles is fast, high-throughput and multi-parameter is still flow analysis. Flow cytometry relies mainly on scattered light or fluorescence to set analytical thresholds and gate analysis. However, since the exosome microbubbles are much smaller than the cells, the scattered light signal is weak, and the conventional flow scattered light detection sensitivity is low, and the exosome microbubbles can be analyzed by means of fluorescent labeling. Among them, non-specific fluorescent labeling is a common method for analyzing the total amount of exosomes.
Recently, de Rond et al. systematically studied the ideal markers for exosome microbubble non-specific markers, and analyzed Calcein AM, Calcein violet, CFSE, Di-8-ANEPPS and Lactadherin in detail (in addition, Annexin V and Lactadherin also bind membrane phospholipids). The composition, and depends on calcium ions, can not be applied to direct labeling of samples such as blood, membrane phospholipid binding efficiency is much lower than Lactadherin, so it is not within the scope of this study) and other types of markers. Because of the different efficiencies of exosome microbubbles marked by different markers, and the different sensitivity to protein aggregates, lipoproteins, cell debris and other substances, the comparative analysis of their efficacy requires very strict comparison information. De Rond et al. first used Apogee nanoflow ultra-high scattering light sensitivity and resolution as a reference standard for different marker labeling efficiency analysis, combined with MCF7 cell supernatant and platelet exosomal microbubble samples, and compared the above five kinds in detail. Markers identify the efficacy of exosomal microvesicles.
Research statistics show that Di-8-ANEPPS, Lactadherin and ultrasensitive scattering light can detect 100% of EpCAM+ MCF7 exosomal microbubbles (Fig. 1); Lactadherin and ultrasensitive scattered light can detect 33% and d 61%, respectively. Platelet CD61+ exosomal microvesicles (Figure 2). Di-8-ANEPPS can specifically recognize the binding of exosomal microvesicles only after removal of the platelet protein journal. Calcein AM, Calcein violet and CFSE are markers for detecting poor sensitivity or cluster detection problems. Therefore, as of now, it has not been able to simultaneously satisfy: labeling all exosomal microvesicles with high specificity, compatibility with antibody markers, direct labeling for biological fluid samples, and labeling non-specific markers with detection time below 8 hours. However, this study also reflects the selection of suitable fluorescent markers for different exosomal microbubble samples, combined with Apogee nanofluid ultrasensitive scattering light, which will still be the best choice for current exosome microbubble flow analysis. Fast, precise and reliable.
Figure 1. EpCAM+ MCF7 exosome microbubble scattered light and fluorescent markers for logistic analysis
Figure 2. Flow cytometry analysis of platelet exocytosis microbubble scattered light and fluorescent markers
references
De Rond, Leonie, et al. "Comparison of Generic Fluorescent Markers for Detection of Extracellular Vesicles by Flow Cytometry." Clinical chemistry 64.4 (2018): 680-689.
Welcome to the official WeChat (micro signal: Bio-Gene)
Public number reply: apogee , watch related technology lecture video
Hong Kong Bo Qi Technology Co., Ltd.
Bio-Gene Technology Ltd.
Guangzhou Boqi Biological Technology Co., Ltd.
400 600 9639
Hong Kong Beijing, Shanghai, Guangzhou, Chengdu, Wuhan, Jinan
The fruit, corn, contains a lot of magnesium, which is known to be one of the few trace elements in the food and can help prevent cancer development.And the fruit corn contains a substance called glutathione, which can make some chemical carcinogens in the body toxic.To ward off cancer.
Although corn is highly nutritious, it is rich in crude fibre, so for children with delicate stomachs, too much of it can easily cause indigestion. If you add milk to the juice and then give it to your child, it will be less taxing on the stomach and more nutritious, and your child will be able to eat more food. (This method uses a wall breaker, but if you don't have one, a juicer is fine.)
If you would like to work with us, please send us a message on the website or contact us by email.
Delectable Sweet Corn Kernel,Sweet Corn Cob Kernels,Storing Fresh Corn Kernel,Baked Sweet Corn Kernels
Jilin Province Argricultural Sister-in-law Food Co., Ltd. , https://www.nongsaocorn.com