Recently, Academician of the Chinese Academy of Engineering, Peking University’s Third Hospital Qiao Jie’s research team and Peking University’s School of Life Sciences Professor Tang Fuhong’s research team collaborated to publish a pre-implantation embryonic multiomics study online in the journal Nature Cell Biology. New Progress - "Single-cell multivariate sequencing of human early embryos". This study used the world's leading single-cell COOL-seq technology to map the genome-wide DNA methylation and chromatin state of human preimplantation embryos for the first time at the single-cell level, and analyzed the complex and coordinated stages of embryonic development. Epigenetic reprogramming process.
This study aims to further reveal the dynamic remodeling process of chromatin status during DNA methylation reprogramming, the specific chromatin status of parents, and the relationship between multiomics. By using single-cell multiomic sequencing technology to distinguish between euploid and aneuploid cells, the single cell data of euploid embryos are used to systematically depict several key stages of human preimplantation embryonic development. Viewing the dynamic changes at multiple levels of the genome has deepened the understanding of the epigenetic reprogramming process of human embryos.
This study revealed for the first time the asymmetric distribution of DNA methylation and chromatin status of the parental genome during the early human embryonic development. The study quantitatively compared the chromatin status of human and mouse embryos and found epigenetic features of species conservation and specificity. This provides a theoretical basis for people to continue to study the similarities and differences of epigenetics in human and mouse early embryos, and clarifies the advantages and limitations of using mice as a model organism to study the early development of mammals. The research provides new ideas and research methods for investigating the mechanism, diagnosis and treatment strategies of embryonic development arrest, implantation failure, recurrent miscarriage and other clinical problems.
Dr. Li Lin, Ph.D., Ph.D. student of Peking University Beijing Advanced Genetic Diagnosis Center, Ph.D. Gao Yun, Guo Fan, researcher of Sichuan University, was the first author of the paper; Qiao Jie and Tang Fu. For the common correspondence author. (Specialist Yang Dongping)
Nucleic Acid (DNA/RNA) Extraction Kit
1. Introduction
The total viral nucleic acid extraction kit is suitable for extracting total viral nucleic acid from serum, plasma, tissue homogenate and other samples. The kit is based on silica column purification technology, which eliminates the need for toxic phenol-chloroform extraction and time-consuming alcohol precipitation. This product has successfully extracted nucleic acids from hepatitis B A/C, hepatitis C, and norovirus standard. The obtained DNA/RNA can be directly used in a series of downstream experiments such as PCR, RT-PCR, and LAMP.
Notice:
1. The carrier RNA solid must be dissolved in Nuclease Free Water to 1µg/µl before use, and vortex to dissolve. Store in aliquots at -70°C. If you need to store it at -20℃ for a long time, please repackage it according to the number of times of use.
2. Dissolve Proteinase K (20mg/ml): Add Proteinase Dissolve Buffer to dissolve Proteinase K to a final concentration of 20mg/ml. Proteinase K dry powder can be stored at 2-8°C for one year, but dissolved Proteinase K must be stored in aliquots at -20°C. Repeated freezing and thawing of Proteinase K can affect its activity.
3. Buffer VHB must be diluted with 14 ml absolute ethanol before use and stored at room temperature.
4. Buffer RW2 must be diluted with 80 ml of absolute ethanol before use and stored at room temperature.
3. Shelf life
Except for Proteinase K and Carrier RNA, other components of this product can be stored at room temperature (15-25°C) for 12 months, and should be stored at 2-8°C for long-term storage. Proteinase K and Carrier RNA dry powder are transported at room temperature. Please store at -20°C after receiving the test product, and store at -20°C after dissolving.
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