Mouse anti-rabbit PAR-1 monoclonal antibody solution product manual (Chinese version)
The main purpose
The mouse anti-rabbit PAR-1 monoclonal antibody solution is an anti-rabbit PAR-1 antibody protein solution intended for use in antigen-antibody immunoreaction experiments. Mainly used in Western hybridization, immunoprecipitation, immunohistochemistry, ELISA and other experiments. The product is ready to use, stable performance and guaranteed activity.
technical background
Protease activated receptor (PAR) belongs to the family of G protein-coupled receptors and contains seven transmembrane proteins, mainly in platelets, endothelial cells, cardiomyocytes and nerves. Highly expressed in the Yuan. The protease activating receptor is divided into four receptors, PAR-1, 2, 3 and 4. PAR-1 is called thrombin receptor; PAR-2, 3, and 4 are called thrombin receptor-1, 2, and 3, respectively. . Among them, PAR1/3/4 is activated by the action of thrombin, and PAR-2 ​​is activated by trypsin. PAR is involved in the regulation of thrombus response and acts on platelet activation, vascular development, pro-inflammatory responses, and the like. The epitope of mouse anti-rabbit PAR-1 monoclonal antibody is located at the N-terminus of PAR-1 and purified by protein G affinity chromatography. Purity More than 95%, molecular weight 20KDa, antibody type is IgG.
product content
Antibody solution (Reagent A) (1 μg/μl) 100 μl
Product manual 1 copy
storage method
Store in a -20 ° C refrigerator to avoid repeated freezing and thawing, effectively guaranteeing June
Experimental tips
- Western hybrid: 1:100 to 500
- Immunohistochemistry: 1:100 to 500
- ELISA: 1:500 to 1000
- Users can adjust the dosage or concentration according to actual needs.
- If you need to dilute, you can use 10 millimolar PBS, pH 7.4 solution
Precautions
- This product is 100 microliter (100 micrograms)
- This product is anti-rabbit specific
- Antibody solution (Reagent A) was stored in 10 mM PBS, pH 7.4 solution
- Strictly avoid repeated freezing and thawing; temporarily not used, kept in the refrigerator at -20 °C
- The company provides a series of ready-to-use antibody reagent products
Quality Standard
- This product has been certified to be stable.
- This product has been identified and is highly specific
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