M4 applied to hydrogel cell observation
The application of engineering science and life science methods to construct artificial structures to guide tissue reconstruction has attracted more and more attention. The common strategy of tissue engineering is to separate specific cells from small pieces of living tissue of patients, under precisely controlled culture conditions. The cells are grown in a three-dimensional porous scaffold, expanded to form a structure, and then the cell/scaffold structure is implanted into a desired part of the body, causing the new tissue to be completed in the scaffold, and the scaffold gradually disappears with the formation of the tissue, and the damage is Organs or tissues can be reconstructed, and biomaterial scaffolds serve as the mechanical support for cell growth and stem cell differentiation into specific cells. Poly(lactide-co-glycolide) and poly(lactic-co-glycolic acid) were first approved for use in the body by the US Food and Drug Administration (FDA), but due to their demanding environmental harshness and disadvantages The loading of growth factors and the retransmission of living cells, so now highly hydrated polymer materials - hydrogel research is fiery.
Hydrogels are constructed of a hydrophilic polymer network that can be cross-linked with biomacromolecules through physical bonds and chemical bonds, and can be formed in a mild environment. The mechanical properties and structural properties of hydrogels are similar to those outside the cell. Matrix and can naturally degrade over a period of time. The most commonly used research materials in tissue engineering, translational medicine, cardiovascular research and biomaterial research.
Difficulties in the research process?
In the research of cell and cardiovascular research using hydrogels, specific cells or stem cells are cultured in a hydrogel for 3D, and the composition and concentration of the hydrogel, the swelling, and the cross-linking method are continuously Exploring, to determine which type of hydrogel is the most suitable? Since it is cultured in 3D matrix, it is impossible to observe and detect the growth state of cells in real time. It is necessary to observe the whole gel material by fluorescence staining. In fact, the photometric property of the fluorescent dye is very strong, and the cell will have a lethal effect for a long time. Morphological observations such as cell extensibility and cell growth can only be performed by laser confocal layer-by-layer scanning. The 3D morphological images of the cells are synthesized and the morphological parameters are calculated by the image software, which is time consuming and cumbersome. It is necessary to explore a number of experimental conditions in the experimental process. Therefore, it is particularly urgent to find an intuitive, convenient and accurate method for detecting cell growth state and cell morphology parameters in cell research in hydrogel.
Technical advantages of HoloMonitor M4 for cell observation of hydrogel materials
In March 2017, we were in the research group of Professor Xu Feng from the School of Life Sciences of Xi'an Jiaotong University. The research group mainly analyzes the growth of cells and the calculation of cell volume at different times. Therefore, we performed real-time monitoring of hydrogel cells of different materials for 24 hours, including 2D culture of hydrogel materials with different thicknesses, 2D culture observation of hydrogels of different thicknesses, and different growth factor treatment hydrogels 3D. Culture observation.
Observation on 2D culture of hydrogel materials with different thickness
Observation of 2D culture of hydrogel materials with different materials
Observation on 3D culture of hydrogels treated with different growth factors
HoloMonitor M4 laser holographic cell imaging and analysis system can detect 2D and 3D of hydrogel for a long time, real-time analysis of cell growth state and morphological parameters of hydrogel, 3D culture in hydrogel In the M4, the multi-layer cells in the gel can be clearly observed, and as many cells as possible within the focal length can be clearly focused and photographed.
Intermediate – Intermediate: A material produced during a drug substance processing step that must undergo further molecular modification or refinement to become a drug substance. Intermediates may or may not be isolated. Intermediates are the key products of the previous process of making APIs, which are different in structure from APIs.
for example:
Amoxicillin capsules are called preparations, amoxicillin is called API, and 6-APA is called intermediate.
Ceftriaxone sodium powder is called preparation, sterile ceftriaxone sodium is called API, and 7-ACA is called intermediate.
Our company specializes in providing key intermediates for various APIs
Intermediate is a very important type of fine chemical products. Its essence is a type of "semi-finished product", which is an intermediate product in the production of certain products. For example, if a product is to be produced, it can be produced from intermediates, saving costs and reducing It is widely used in the synthesis of medicines, pesticides, coatings, dyes and spices. In the field of medicine, intermediates are an important link in the industrial chain of the pharmaceutical industry. They are used for some chemical raw materials or chemical products in the process of drug synthesis. In a word, they are used to produce APIs.
Key Intermediates , Pharmaceutical intermediates , Anticancer intermediates , Antitumor Intermediates
XI AN RHINE BIOLOGICAL TECHNOLOGY CO.,LTD , https://www.xianrhinebiotech.com