First, the surface disinfection is divided into skin surface disinfection and disinfection of the surface of utensils. 1. The surface of the skin is generally disinfected with the following two commonly used disinfectants (1) The best concentration of 70% - 75% ethanol concentration. The 95% alcohol, which is generally sold on the market, needs to be prepared with distilled water. The preparation method is to add 20mL of distilled water to 75mL of 95% alcohol. (2) Bezoar is a commonly used aqueous solution with a concentration of 0.25%. Do not contact with aluminum or other metal products to prevent corrosion and rust. Use with anionic detergents. Do not use with peroxide disinfectants. Short duration of sterilization. 2. Disinfection of the surface of the vessel In addition to 70% - 75% of the more commonly used alcohol, there are the following. (1) Sulfur Soap Liquid contains 42%-50% of viscous liquid with phenol odor. 3% of the solution is commonly used for surface disinfection of containers. (2) Chlorhexidine is generally sold at a 5% chlorhexidine solution, and the sterilizer needs a concentration of 0.5% to 1.0%. (3) Bleach General commercial bleaching powder containing 25% - 30% chlorine, with a pungent odor. Commonly used 2% - 5% solution as a surface disinfectant. (4) The usual concentration of potassium permanganate is 0.1%-0.2%. Second, there are many kinds of space disinfection disinfectants, which are commonly used as follows. 1. Ultraviolet light irradiation UV light irradiation distance should not exceed 1.2 meters. In order to increase the disinfection effect, the interior can be sprayed with 5% stone carbonate solution or other disinfectant before turning on the lamp, and then turn on the lamp for 30 minutes. The operator should not work under ultraviolet rays, nor should he look directly at the ultraviolet rays. 2.Formaldehyde and potassium permanganate must be insufflated in the inoculation room or inoculation box before fumigation. Generally, 10 mL of formaldehyde per cubic meter, and 4-5 grams of potassium permanganate are placed in the tray, and the mixture of the two produces gas. . The inoculation room should be sealed for 24 hours to achieve the disinfection effect. The inoculation box can be sealed for 1-2 hours. 3. Sulfur light yellow solid, sulfur dioxide generated when burning, sulfur dioxide in water vapor to generate sulfurous acid, sulfuric acid adhered to the bacterial cell, to seize the oxygen in the cells to deoxidize the bacteria cells and die. Space disinfection generally uses 20 grams of sulfur per cubic meter, and is placed in a plate to ignite. In order to increase the burning effect, some wood chips or alcohol can be added to the plate, and the sterilizing effect can be achieved within 24 hours. To enhance the effect, water should be sprayed into the room to increase the air humidity. It is often used interchangeably with other disinfectant drugs to prevent drug-resistant bacteria. 4. Phenol is also known as phenol or stearic acid, white crystals, commonly used concentration 5%, for spray disinfection of inoculation room or inoculation box. As phenol has a corrosive effect on the skin, it should be kept in a dark place.
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